Self Evaluation

Q1. Enzymes are -
A    Very specific towards reaction with substrate
B    Effective at low concentrations
C    Unchanged in the reaction
D    Known to feature all the above properties

Q2. The velocity of enzymatic reaction (V) -
A    Can be calculated by either calculating rate (dS/dt) or rate (dP/dt)
B    Can be calculated by rate dP/dt only
C    Can be calculated by rate dS/dt only
D    Is calculated by a correlation between dS/dt and dP/dt"

Q3. Michaeli’s Menten equation is described by the following equation -
A    V = Vmax S / (Km +S)
B    V = Vmax Km / (Km +S)
C    V = (Km +S) / Vmax S
D    V = Vmax S + Km S / Vm

Q4. The assumption of Michaelis Menten equation are - (i)Enzyme (E) & Substrate (S) reacts
      to form Enzyme –Substrate (ES) complex. (ii) ES complex breaks down to yield Enzyme
    (E) & Product (P). In above reactions indicate which of the following statements are
            true -
A    Reaction (i) is fast reversible reaction while reaction (ii) is slow reaction. Out of the
            two reactions rate of reaction (ii) decides the overall velocity of reaction
B    Reaction (i) is slow reversible reaction whereas reaction (ii) is fast reaction. Out of
            the two reactions, rate of reaction (i) decides the overall velocity of reaction
C    Reaction (i) is fast irreversible reaction whereas reaction (ii) is fast reversible
            reaction. Out of the two reactions, rate of reaction (ii) decides the overall velocity of
            reaction
D    Both reactions proceed in an irreversible manner and contribute in the estimation of
            velocity of reaction

Q5. Maximum velocity of enzymatic reaction -
A    Can be calculated from ‘V’ vs ‘S’ curve
B    Can be calculated from ‘1/V’ vs ‘1/S’ curve
C    Is asymptote to ‘V’ vs ‘S’ curve
D    All of the above is true

Q6. ‘1/V’ vs ‘1/S’ curve -
A    Is called as Lineweaver Burk Plot
B    Slope of above curve is given by ‘Km/Vm’ and intercept is ‘1/Vm’
C    Yields accurate values of ‘Vm’ & ‘Km’ than ‘V’ vs ‘S’ plot
D    All of the above is true

Q7. Affinity of substrate towards enzyme -
A    Inversely proportional to Km value
B    Is higher at higher Km value
C    Is not dependent on Km value
D    Is directly proportional to Km value

Q8. Michaeli’s Menten constant Km -
A    Is defined as the concentration of substrate at which V = Vm / 2
B    Is defined as the concentration of substrate at which V = Vm
C    Is defined as the concentration of substrate at which V = Vm /3
D    Is defined as the concentration of substrate at which V = Vm /4

Q9. Units of ‘Vm’ & ‘Km’ are -
A    g/L-h & g/L respectively
B    g/L & g/L-h respectively
C    g-l/h & g/L respectively
D    None of the above is true

Q10. Turn over number of enzyme is defined as -
A    Number of moles of substrate converted by one mole of enzyme per minute
B    Number of gms of substrate converted by one mole of enzyme per minute
C    Number of gms of substrate converted by one gm of enzyme per minute
D    None of the above is true