Immobilization of whole cells
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Self Evaluation
Q1. The main advantages of immobilization are
A
Cost reduction of the process
B
Reusability of the enzymes
C
Elimination of isolation steps of purification of enzymes
D
All of the above
Q2. The main merits of immobilization of enzyme are
A
The enzyme can be reused
B
It eliminates contamination problem
C
It helps in improvement of productivity of product
D
All of the above
Q3. The immobilization of “whole cell”
A
Ensures availability of co-factor essentially required for certain enzymatic reactions
B
Eliminates expensive & cumbersome purification protocols for isolation of enzymes.
C
Features same protocols for immobilization
D
All of the above
Q4. The main techniques of immobilization are
A
Adsorption
B
Microencapsulation
C
Covalent binding
D
All of the above
Q5. The apparent Km of the immobilized enzyme will
A
Invariably increase with immobilization
B
Invariably decrease with immobilization
C
Remain same with immobilization
D
None of the above
Q6. The pH profile the immobilized enzyme will
A
Remain same as that of the free enzyme
B
Will be different as compared to free enzyme
C
Will change depending on the charges of the carrier
D
None of the above is true
Q7. The stability of the immobilized enzyme will
A
Increase by immobilization
B
Decrease by immobilization
C
Not change by immobilization
D
None of the above is true
Q8. The major problem of immobilization by “alginate” method is
A
The beads of immobilized enzymes get dissolved in the presence of phosphate
B
The beads of immobilized enzymes feature severe diffusion problem of substrate
C
The beads of immobilized enzymes are too fragile in nature
D
None of the above is true
Q9. The main bio-reactors used for enzymatic reactions are
A
Stirred tank reactors
B
Column reactors
C
Membrane reactors
D
All of the above
Q10. The “Reactor space time” of an immobilized enzyme reactor is defined as
A
VR /Q where ‘VR ‘is the volume of the reactor and ‘Q’ is the flow rate through the reactor
B
VR x Q where VR is the volume of the reactor and Q is the flow rate through the reactor
C
Q/ VR where VR is the volume of the reactor and Q is the flow rate through the reactor
D
Q2/ VR where VR is the volume of the reactor and Q is the flow rate through the reactor