Immobilization of whole cells

Self Evaluation


Q1. The main advantages of immobilization are
A    Cost reduction of the process
B    Reusability of the enzymes
C    Elimination of isolation steps of purification of enzymes
D    All of the above

Q2. The main merits of immobilization of enzyme are
A    The enzyme can be reused
B    It eliminates contamination problem
C    It helps in improvement of productivity of product
D    All of the above

Q3. The immobilization of “whole cell”
A    Ensures availability of co-factor essentially required for certain enzymatic reactions
B    Eliminates expensive & cumbersome purification protocols for isolation of enzymes.
C    Features same protocols for immobilization
D    All of the above

Q4. The main techniques of immobilization are
A    Adsorption
B    Microencapsulation
C    Covalent binding
D    All of the above

Q5. The apparent Km of the immobilized enzyme will
A    Invariably increase with immobilization
B    Invariably decrease with immobilization
C    Remain same with immobilization
D    None of the above

Q6. The pH profile the immobilized enzyme will
A    Remain same as that of the free enzyme
B    Will be different as compared to free enzyme
C    Will change depending on the charges of the carrier
D    None of the above is true

Q7. The stability of the immobilized enzyme will
A    Increase by immobilization
B    Decrease by immobilization
C    Not change by immobilization
D    None of the above is true

Q8. The major problem of immobilization by “alginate” method is
A    The beads of immobilized enzymes get dissolved in the presence of phosphate
B    The beads of immobilized enzymes feature severe diffusion problem of substrate
C    The beads of immobilized enzymes are too fragile in nature
D    None of the above is true

Q9. The main bio-reactors used for enzymatic reactions are
A    Stirred tank reactors
B    Column reactors
C    Membrane reactors
D    All of the above

Q10. The “Reactor space time” of an immobilized enzyme reactor is defined as
A    VR /Q where ‘VR ‘is the volume of the reactor and ‘Q’ is the flow rate through the
            reactor
B    VR x Q where VR is the volume of the reactor and Q is the flow rate through the
            reactor
C    Q/ VR where VR is the volume of the reactor and Q is the flow rate through the
            reactor
D    Q2/ VR where VR is the volume of the reactor and Q is the flow rate through the
            reactor