Self Evaluation

Q1. Fed-batch cultivation :
A    Does not eliminate substrate inhibition
B    Reduces the time of cultivation
C    improves the productivity of microbial cultivation
D    eliminates product inhibition

Q2. Constant feeding of substrate in model based fed-batch cultivation may result in:
A    Ease of experimental implementation
B    Improved product accumulation
C    accumulation of substrate concentration higher than initial concentration of batch
D    All of the above

Q3. Linear feeding of substrate in model based fed-batch cultivation may result in:
A    Requirement of computer interfaced peristaltic pump
B    Matching of growth with the substrate feeding and thereby constant substrate
            availability in the reactor
C    Decrease in productivity of the process
D    A & B

Q4. Decreasing feeding of substrate in model based fed-batch cultivation may result in:
A    Filling of reactor and termination of cultivation at the same time
B    Improved productivity of the process
C    Enhanced cultivation time
D    A, B & C

Q5. Model based off line feed addition strategy in fed-batch cultivation:
A    Helps in improvement of productivity of process by minimum experiments
B    Can feed the Carbon, Nitrogen sources only when exhausted and thereby avoiding
            inhibition due to them
C    Can design the substrate inhibition free cultivations
D    All of the above

Q6. For a ideal feeding strategy of fed-batch cultivation one should consider:
A    that Substrate inhibition is eliminated
B    the maximum reactor volume is used for fresh nutrient addition
C    the substrate concentration should be zero and product concentration maximum when
            the reactor is full
D    All of the above

Q7. Monod’s model assumes that:
A    All the cells are identical in the bioreactor
B    All the cells are homogenously distributed in the reactor
C    The cell is like a black box and nothing is known about its intracellular reactions
D    All of the above

Q8. Monod model:
A    Is an unstructured model
B    It does not describe the lag phase
C    It may not predict reality under highly dynamic conditions
D    Both A & C

Q9. The cells may stop growing in the bioreactor even under excess limiting nutrient
       (substrate) conditions:
A    This may be because of high product accumulation which is inhibitory
B    This may be due to development of the mutant strain of the culture
C    This may be due to shearing of the cells due to high RPM &/or oxygen
D    Both A & C

Q10. Following is important while deciding the substrate concentration in the feed bottle in
         the model based fed-batch cultivation:
A    The Substrate should be kept as high as possible to in order to avoid quick filling of
            the reactor during feed addition
B    Substrate concentration and its rate of addition both are appropriately selected to get
            best productivity from fed-batch cultivation
C    A & B
D    None of the above