Self Evaluation

Q1. Batch animal cell cultivation has following problems:
A    Severe inhibition of accumulated products
B    High concentration of unconverted substrate
C    Long cultivation time
D    Both a and b

Q2. The fed-batch cultivation:
A    Dilutes the accumulated products and reduce inhibition of products
B    Enhance substrate and nutrient concentration in broth
C    Reduce the death of culture for some time thereby increasing longevity of cell and
            in-term more product formation
D    All of the above

Q3. Model based design of fed-batch cultivation:
A    Features process optimization with minimum trial experiments
B    Usually fails as the mostly the models are empirical in nature
C    Generally helps in prediction of fed-batch kinetics
D    Both a & c

Q4. For preparation of animal cell cultivation in the bioreactor:
A    Physiological solution is added in the reactor and DO probe calibrated after
            sterilization
B    The reactor is sterilized by Chemical Spray
C    The reactor is autoclaved with media in it
D    The reactor is blank sterilized and autoclaved medium is pumped aseptically In the
            laminar hood

Q5. Fed-batch cultivation is usually effective in:
A    Cultivations having substrate inhibition
B    Cultivations have product inhibition
C    Cultivations have both substrate and production inhibition
D    None of the above

Q6. Fetal Calf Serum in animal cell cultivation:
A    Is an essential component for good growth and product formation
B    Should be sterilized by autoclaving only
C    Should be sterilized using filters
D    Both a & c

Q7. The size of animal cell:
A    Is smaller than a microbial cell
B    Is bigger than a microbial cell
C    Is equal to that of microbial cell
D    None of the above

Q8. To meet the high requirement of oxygen in animal cell cultivation:
A    It may be necessary to purge air from the sparger
B    It may be necessary to purge air in head space
C    It may be necessary to do bubble free aeration by dialysis tubing
D    Both b & c

Q9. The specific growth growth rate of animal cell:
A    Is dependent on availability of both limiting nutrient glucose and glutamine
B    Is dependent on availability of not only glucose and glutamine but also depends
            onconcentrations of lactate and ammonia.
C    Is dependent on the availability of glutamine and lactate.
D    Is dependent on the availability of only glucose and ammonia.

Q10. The inoculum volume for animal cell is primarily calculated:
A    Based on the initial live cells required in the final vessel
B    Based on total number of cells required in the final vessel
C    Based on cell mass of inoculum
D    Based on either b or c