Self Evaluation

Q1. Animal cell –
A    Do not have Cell wall
B    Have thick cell wall
C    Have high doubling time
D    None of the above

Q2. The live and dead cell count in animal cell is done by –
A    Tryphan blue staining
B    Methylene blue staining
C    Gram staining
D    Woolford stain

Q3. The sterilization of animal cell culture media is done by -
A    Filter sterilization
B    Autoclaving
C    Dry sterilization
D    Addition of chemical agents

Q4. The two substrates needed for the growth of Animal cell cultivation are –
A    Glucose and glutamine
B    Glucose and sucrose
C    Glucose and fructose
D    Glucose and maltose

Q5. The bye products produced in animal cell cultivation are –
A    Lactate and ammonia in which lactate is more inhibitory
B    Lactate and pyruvate in which pyruvate is more inhibitory
C    Lactate and ammonia in which ammonia is more inhibitory
D    Lactate and butyrate in which lactate is more inhibitory

Q6. The air supply in the animal cell cultivation is facilitated by –
A    Head space sparging
B    By purging air from the sparger
C    By either a or b
D    None of the above

Q7. The pH control in the animal cell cultivation is done by –
A    Acid and alkali addition
B    Ammonia and CO2 purging
C    By either of above procedures a or b
D    None of the above

Q8. The requirement for growth of animal cells are –
A    37° C and purging of 5 % CO2
B    37° C and purging of 2 % CO2
C    30° C and purging of 5 % CO2
D    30° C and purging of 2 % CO2

Q9. For prevention of contamination the animal cell cultivation –
A    Is done in Laminar Hood
B    5 % Gentamycin is added in the media
C    5 % alcohol is added in the media
D    5 % formaldehyde is added in the media

Q10. The bioreactor used for animal cell cultivation generally has –
A    Flat blade turbine Impeller
B    Setric Impeller
C    Either of a and b
D    No impeller