Plant Cell-Batch Cultivation | ||||||
Objective: To initiate callus cell line of A. indica, develop suspension culture & study its growth and product formation kinetics under batch cultivation conditions in bioreactor. Theory: Basic concepts & need of plant cell cultivation Plants have provided us with food, fuel and fibers since prehistoric times. They have been inexhaustible source of a diverse array of chemicals such as flavors, fragrances, natural pigments, pesticides and pharmaceuticals. This seemingly unrelated collection of chemicals has been grouped together under a broad category of plant secondary metabolites, although many of them are known to be produced during active growth phase of the plant cells (DiCosmo and Misawa, 1995). The area which has received maximum attention from scientific community is the potential use of plant-derived compounds as a source of medicines, aromas and fragrances. Currently these compounds are being produced by cultivation of the whole plant followed by solvent extraction. This conventional process presents a number of disadvantages such as non-availability of the source plant throughout the year, risk of extinction of the plant species due to its mass collection and inconsistent product yield due to climatic variations. Hence, in vitro cultivation of plant cells in bioreactor is considered to be an attractive alternative and viable approach for the production of these compounds. |
||||||
Copyright @ 2015 Under the NME ICT initiative of MHRD (Licensing Terms) |